Isolation and Characterization of Phosphate Solubilizing Microbes from Rock Phosphate Mines and Their Potential Effect for Sustainable Agriculture

Continuous application of phosphate (P) mineral to soil renders apatite addition during each crop growing season which is great concern from a sustainable agriculture viewpoint. Use efficient solubilizing microbes (PSB) one the most effective ways solubilize this in soil. The current study targeted hydroxyapatite mines explore, isolate and characterize P solubilizers Efficiency isolated rock (hydroxyapatite) tri-calcium (TCP) as well indole-3-acetic acid (IAA) 1-aminocyclopropane-1-carboxylate deaminase (ACC) activity were tested. Identification phylogenetic analysis bacterial fungal isolates carried out by 16S rRNA internal transcribed spacer (ITS) rDNA sequence analyses, respectively. strains identified Staphylococcus sp., Bacillus firmus, safensis, licheniformis whereas Penicillium sp. oxalicum. Results showed that impact combination with three fertilizers sources (compost, diammonium (DAP)) was conspicuous on maize grown pot. Both increased uptake plants recorded higher available post-harvested compost resulted maximum P-uptake post-harvest contents, compared other combinations bio-inoculants. Screening can be better option utilize indigenous reserves organic amendments for agriculture.